BD LSRFortessa

Superior Performance

Many innovations are incorporated into the BD LSRFortessa product line. The heart of the cytometer, the fluidics system features a true fixed alignment flow cell that is gel-coupled to the collection optics to maximize detector signal.


Fluidics System

The fluidics system is pressure driven. Hydrodynamic focusing forces sample cells through the cuvette flow cell, where they are interrogated. The flow cell is in fixed alignment with the laser and gel-coupled to the collection optics. This design ensures that the laser is precisely focused on the sample stream and the maximum amount of emitted light can be collected for added sensitivity in multicolor applications. Fixed alignment also minimizes startup time, improves experiment-to-experiment reproducibility, and enables automated daily quality control.

The sheath container (8 L) and waste container (10 L) are outside the cytometer, and positioned on the floor for easier access.

The optional BD FACSFlow™ supply system fluidics cart increases capacity and ease of use while maintaining a stable fluidics pressure. It includes an automated sheath and waste fluid control system that reduces daily maintenance by incorporating two 20-L containers (Cubitainers®).

Fluidic sensors maintain constant pressure, while a fluidics monitoring system warns when sheath fluid is low, empty, or when the waste container is full.

Cuvette flow cell
Cuvette flow cell

A quartz cuvette flow cell is gel-coupled to the collection optics. This design helps ensure that lasers are precisely focused on the sample stream to generate the greatest signal and ensure that the maximum amount of emitted light is collected.

Hydrodynamic focusing of the sample core through the flow cell
Hydrodynamic focusing of the sample core through the flow cell