Stem cell transplantation with cell selection is performed after high-dose chemotherapy (HDC) in an effort to restore a cancer patient’s blood and immune cell production capacity, which is destroyed in this aggressive form of cancer treatment. Improvements in transplantation techniques, including the wider use of cell selection, have also contributed to a significant reduction in the morbidity and mortality associated with conventional transplantation. As a result, HDC with transplantation has grown at an average annual rate of 21% over the past 10 years. HDC with transplant is now standard therapy in multiple myeloma, acute myeloid leukemia in first remission, and intermediate-grade non-Hodgkin's lymphoma.

BD Stem Cell Enumeration Kit

The BD™ Stem Cell Enumeration Kit offers a single tube, single platform assay for accurate, reproducible and rapid enumeration of CD34+ hematopoietic and progenitor cells in line with ISHAGE-based protocol guidelines. The kit works with a wide range of stem cell specimens including normal and mobilized peripheral blood, fresh and post-thaw apheresis products, fresh and post-thaw cord blood, fresh and post-thaw bone marrow samples. The kit includes BD Trucount™ technology to improve test accuracy, eliminating the need for manual pipetting of counting beads. The kit uses one test tube per sample and does not require an isotype control, improving operational efficiency.

BD Procount Progenitor Cell Enumeration Kit

The BD Procount™ Progenitor Cell Enumeration kit is a two-tube assay to identify and enumerate absolute counts and percentages of CD34+ cells in human normal and mobilized peripheral blood samples and leukapheresis samples using flow cytometry. The kit includes the BD Trucount technology to improve test accuracy and eliminate manual pipetting of counting beads. The CD34 reagent contains nucleic acid dye for DNA and RNA staining of all nucleated cells and CD34 PE-labeled monoclonal antibody. The kit also includes an isotype control reagent. The BD Procount™ software runs on the BD FACSCalibur™ and allows both automated CD34+ analysis or ISHAGE-type manual analysis using sequential gating.