Innovations and Fail-Safes Deliver Stability from Day to Day, All Day

Based on extensive experience developing and supporting high-speed cell sorters, the BD FACSJazz delivers operational efficiency and high performance. The stability of the fluidics system provides reliable sorting performance from day to day, all day long. A number of system features ensure a trouble-free sorting experience—empowering quick and easy sort setup and operation.

Acoustical coupling in the nozzle assembly

The fluidics system in the BD FACSJazz uses a highly efficient acoustical coupling in the nozzle assembly to create stable droplets at a moderate sheath pressure. These settings optimize cell viability, which can be critical to maximizing cell yield from sorting. To further ensure simple operation, the BD FACSJazz fluidics system has been designed without in-line valves or components in the sheath and sample fluidic path.

Novel pressure console and software control

A novel pressure console design uses digital sensors to control pressure. The pressure console is integrated with BD FACS Sortware software to control all fluidics functions. The software automates basic functions for sample loading, and coordinates functions such as opening valves, boosting, and running the sample to start a sorting experiment. When the BD FACSJazz is in a biological safety cabinet, this comprehensive software control enables the operator to remotely control the fluidic functions, minimizing the operator’s direct interactions with the cytometer.

Nozzle assembly

The nozzle tip is mechanically coupled to the system using a threaded nut, allowing the tip to be removed for cleaning without grossly impacting system alignment. This allows researchers to have greater control with greater ease of use.

Exchangeable fluidics
Exchangeable fluidics
An important concern in cell sorting is crosscontamination, either between samples or with foreign biological agents such as bacteria, viruses, and DNA. To mitigate the risk of cross-contamination, the sample path is free of in-line valves to minimize contact between cells and non-replaceable parts. For absolute and rigorous control, the complete fluidics path, including all parts that potentially contact cells, can be replaced with a disposable fluidics kit.
Pressure console controls
Pressure console controls
Murine stem cell development progression
Murine stem cell development progression
BD FACSJazz™ analysis of murine stem cells expressing GFP with Flk1 reveals three distinct cell populations, GFP-Flk1-, GFP+Flk1-, and GFP+Flk1+cells, which represent a developmental progression ranging from pre-mesoderm to pre-hemangioblast mesoderm to the hemangioblast. GFP expression is a marker for mesoderm. The antibody used was Flk1 PE-Cy™7.
Data courtesy of Valerie Kouskoff, Stem Cell Haematopoiesis Group, Cancer Research UK, Paterson Institute for Cancer Research, The University of Manchester, England.

Streamlined operation

To simplify operator experience, the 100-micron nozzle tip can be easily removed and replaced. In addition, other fail-safe features in the fluidics system also help enable a simplified operator experience. This includes a bubble detector built into the fluidics system to automatically stop the flow when a sample tube is empty to prevent air bubbles from reaching the nozzle and compromising sort performance. Simple keyboard shortcuts such as sample, boost, and run, enable keyboard control of the fluidics system to further simplify operation.

Sheath and waste tanks

The BD FACSJazz is equipped with 7-L stainless steel sheath and waste tanks. When instruments are used with BSCs, the tanks may be located outside the containment area. The waste tank remains sealed under vacuum and does not generate aerosols. Bleach can be added to the waste tank to inactivate biological materials to ensure that they do not pose a risk to operators.

Cy™ is a trademark of Amersham Biosciences Corp. Cy dyes are subject to proprietary rights of Amersham Biosciences Corp and Carnegie Mellon University and are made and sold under license from Amersham Biosciences Corp only for research and in vitro diagnostic use. Any other use requires a commercial sublicense from Amersham Biosciences Corp, 800 Centennial Avenue, Piscataway, NJ 08855-1327, USA.