Baker Company Custom Cabinet Meets Biosafety Standards

A biological safety cabinet (BSC) designed specifically for the BD FACSJazz™ by The Baker Company measuring 53.75 x 34.5 x 91.87 to 98.37 in. (136.5 x 87.5 x 233 to 250 cm) is available as an option. Biological safety in flow cytometry is an emerging requirement for core laboratories concerned about the potential accidental exposure of operators to biological samples.

Protecting personnel, products, and the environment

BSCs are designed to protect operators from risks associated with exposure to biological agents in samples. These cabinets are among the most effective and commonly used primary containment devices in laboratories working with infectious agents. BSCs protect personnel and the environment from harmful agents and protect product (cells) from contamination.

Verified to meet biosafety standards

The Baker Company has verified that the BSC designed specifically for the BD FACSJazz cell sorter meets personnel and product protection standards for a Class II Type A2 biosafety cabinet, the National Sanitation Foundation International Standard 49, and European Standard 12469. Importantly, all microbiological testing was performed with the BD FACSJazz placed inside the work area of the BSC to validate performance in an as-used condition.

Airflow control

The BSC controls the direction, volume, and speed of airflow to direct potentially harmful particles away from the operator. Air is filtered and circulated around the work surface, and a separate airflow at the front of the cabinet creates a protective barrier for the operator.

Aerosols from the BD FACSJazz sort chamber are directly evacuated into the high efficiency particulate air (HEPA) filters of the BSC, eliminating the need for an aerosol management option. The HEPA filters remove microorganisms and airborne particulates (aerosols) from the air. Filters are placed where the air enters and exits the work area. Filters are placed where a percentage of the air is exhausted from the cabinet and where the cabinet air is recirculated. HEPA filters in the cabinet support the removal of a minimum of 99.97% of particles or equivalent with a diameter of 0.3 μm.

Isolation of single hematopoietic stem cells from murine bone marrow
Isolation of single hematopoietic stem cells from murine bone marrow

Hematopoietic stem cells were identified in the bone marrow of a mouse expressing eGFP under the actin promotor. Sequential gating by scatter, then of singlets, lineage/c-kit+, Sca-1+, and CD150+CD48 events was used to deposit single GFP+ HSCs into 72-well Terasaki plates with the BD FACSJazz in 1.0 drop purity mode at a rate of 1,200 cells per second. The final plot shows the lineage/c-kit+/Sca-1+ cells back gated on the FSC vs SSC plot.

Antibodies used were CD150 PE, Lineage PerCP-Cy™5.5, Sca-1 PE-Cy™7, and c-Kit APC and CD48 APC-Cy7.

Cy™ is a trademark of Amersham Biosciences Corp. Cy dyes are subject to proprietary rights of Amersham Biosciences Corp and Carnegie Mellon University and are made and sold under license from Amersham Biosciences Corp only for research and in vitro diagnostic use. Any other use requires a commercial sublicense from Amersham Biosciences Corp, 800 Centennial Avenue, Piscataway, NJ 08855-1327, USA.