BD Phosflow Cell Signaling

BD Biosciences provides a comprehensive portfolio of tested antibody reagents, optimized buffers, and robust protocols for conducting phosphoprotein signaling analysis in different sample types.


Flexible analysis with a library of tested phosphoprotein markers

High quality monoclonal anti-phosphoprotein antibodies from more than a dozen signaling pathways provide flexibility for studying complex signaling networks. Additionally, antibodies that recognize either phosphorylated or non-phosphorylated protein states offer multiple analysis permutations.

Strict validation criteria for monoclonal antibodies, including verification by Western blot, optimal signal to noise ratios, and signal blocking by specific phosphopeptides, ensure dependable results and consistency with legacy Western blotting data.

Tested antibodies to surface markers reduce guesswork

BD Biosciences has tested antibodies against cell-surface antigens for resistance to the fixation and permeabilization procedures of BD Phosflow. This continuously expanding library of monoclonal antibodies includes markers for human and mouse leucocyte differentiation and offers access to a wide range of cell types.


Multiple phosphoproteins in different cell types studied by BD Phosflow.

Activation profiles monitored with the BD Phosflow Human Monocyte/NK Cell Activation Kit

Activation profiles of phosphorylated Stat proteins for CD14+ monocytes, CD16/CD56+ NK cells, CD3+ T cells, and CD19+ B cells. Data were collected on a BD FACSCanto™ II flow cytometer and analyzed using Cytobank software. These histograms show the signaling response of each phosphorylation marker induced by the corresponding response modifier

Activation profiles monitored with the BD Phosflow Human Monocyte/NK Cell Activation Kit

Activation profiles of phosphorylated Stat proteins for CD14+ monocytes, CD16/CD56+ NK cells, CD3+ T cells, and CD19+ B cells. Data were collected on a BD FACSCanto™ II flow cytometer and analyzed using Cytobank software. These histograms show the signaling response of each phosphorylation marker induced by the corresponding response modifier.