BD LSRFortessa

Greater Consistency

BD FACSDiva™ software controls the efficient setup, acquisition, and analysis of flow cytometry data from the BD LSRFortessa workstation. The software is common across BD FACS™ instrument families, including the BD FACSCanto™ cell analyzer and BD FACSAria™ cell sorter systems. This affords researchers greater application flexibility allowing them to easily move assays from one platform to another.

Cytometer Setup and Tracking

The Cytometer Setup and Tracking (CS&T) feature of BD FACSDiva software establishes baseline settings and adjusts for instrument variability. The software reduces operator error, and ensures consistency of results by setting the signal time delay across the multiple laser beams and optimizing PMT voltages. Application specific settings can also be set, allowing for rapid setup and performance of routine experiments in a more consistent manner. Quality control (QC) tracking capabilities in the software measure instrument settings and report on performance. Levey-Jennings plots help users understand instrument performance and identify maintenance issues.

Acquisition and Analysis

BD FACSDiva software enables researchers to preview and record data from multiple samples with an automated acquisition process. Acquisition templates, user-definable experiment layouts, and simple compensation procedures are also managed by the software to further facilitate acquisition.

Controls - FACSDiva Worksheet Parsley

BD FACSDiva worksheet showing well defined T-cell population subsets acquired using the special order BD LSRFortessa analyzer configured with the following lasers: 60-mW 355-nm UV, 100-mW 450-nm violet, 100-mW 488-nm blue, and 40-mW 640-nm red.

For analysis, the software includes powerful features such as hierarchical snap-to gating, a variety of plot formats, and batch analysis. Recorded data can be analyzed by creating plots, gates, population hierarchies, and statistical views on a BD FACSDiva global worksheet. Once the global worksheet is saved, it can be used to analyze multiple sample tubes from an experiment, thereby saving time. Numerous other productivity benefits include user-definable batch analysis and automated features such as gate resizing, pausing between data files, exporting statistics, and printing before proceeding to next data file.