세포자연사, 세포주기 및 세포 증식

CD4-enriched mouse splenocytes were cultured with anti-CD3/CD28.
CD4-enriched mouse splenocytes were cultured with anti-CD3/CD28, IL-2, and IL-4 for 6 days. Cells were harvested and treated with 10 ng/mL IL-2 +1 µg/mL colcemid for 4 (left) or 24 (right) hours and stained with the BD Cycletest™ Plus DNA reagent kit.
Cell cycle analysis of a population stained for incorporated BrdU and total DNA levels (7-AAD).
Cell cycle analysis of a population stained for incorporated BrdU and total DNA levels (7-AAD).
Human peripheral blood mononuclear cells (PBMCs) were stimulated with anti-CD3/CD28 for 48 hours and re-stimulated with PMA + ionomycin for 4 hours, and BrdU was added for the final 1 hour. Cells were then harvested and stained using the BrdU staining protocol.
The use of VPD450 to correlate cell proliferation with IL-2 production.
The use of VPD450 to correlate cell proliferation with IL-2 production.
CD4+ enriched mouse splenocytes were loaded with 1 µM of VPD450 for 10 minutes. Cells were then stimulated with anti-CD3/CD28 and harvested at the indicated times. Approximately 4 to 6 hours prior to harvest, cells were stimulated with PMA + ionomycin in the presence of BD GolgiStop™ protein transport inhibitor. Cells were fixed and permeabilized, stained for IL-2, and analyzed on a BD™ LSR II flow cytometer.
Cell cycle analysis of HeLa cells treated with aphidicolin.

Cell cycle analysis of HeLa cells treated with aphidicolin (a DNA polymerase inhibitor) monitored by BrdU staining. The images were captured on a BD Pathway™ 855 bioimaging system with a 20x objective and merged using BD Attovision™ software. Hoechst - Blue, BrdU - Red, Histone H3 (pS28) - Yellow, Tubulin - Green.


 
Radio frequency dose-dependent apoptosis, necrosis, and cell death monitored by Annexin V - BD Horizon V450.
Radio frequency dose-dependent apoptosis, necrosis, and cell death monitored by Annexin V - BD Horizon™ V450 in pancreatic carcinoma cell lines treated with a low dose of cetuximab-targeted gold nanoparticles. As the RF field power increases, the temperature increases, and a shift from apoptosis (lower right quadrant) to frank necrosis (upper left quadrant) is seen.
Flow cytometric analysis of apoptotic and non-apoptotic populations using anti-active caspase-3 antibodies.
Flow cytometric analysis of apoptotic and non-apoptotic populations using anti-active caspase-3 antibodies.

Jurkat T cells (A, A1) or mouse thymocytes (B, B1) were left untreated (A, B) or treated for 4 hours with camptothecin (A1) or a mouse Fas monoclonal antibody, clone Jo2 (Cat. No. 554254) to induce apoptosis (B1). Cells were permeabilized and then stained with PE-conjugated active caspase-3 antibodies (Cat. No. 557091). Untreated cells were primarily negative for the presence of active caspase-3, whereas about half of each population of cells induced to undergo apoptosis had detectable active caspase-3.

In this experiment, Jurkat cells were treated with camptothecin, a potent inhibitor of topoisomerase I and apoptosis inducer.

In this experiment, Jurkat cells were treated with camptothecin, a potent inhibitor of topoisomerase I and apoptosis inducer. Phosphorylation of H2AX, a protein important for maintaining genome integrity, has been shown to correlate with levels of DNA damage. Using multicolor flow cytometry, cell proliferation (BrdU), apoptosis (cleaved PARP), and DNA damage (histone H2AX pS140) were evaluated in the same experiment.



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