BD Accuri News

 

May 2017


Spotlight

Ennio Tasciotti on nanomedicine

Spotlight - Tasciotti

Ennio Tasciotti is an associate professor of nanomedicine at the Institute for Academic Medicine at Houston Methodist Hospital in Texas. His research focuses on developing innovative nanotechnology platforms and biomaterials for drug delivery and regenerative medicine. Dr. Tasciotti spoke to us about the promise of nanomedicine and biomimetic nanoparticles, how his lab uses the BD Accuri™ C6 Plus flow cytometer and how NASA has inspired his research.
Read the interview »

Noteworthy

Join us at the 2017 BD Horizon™ Global Tour

Noteworthy - Horizon Tour

The 2017 BD Horizon Global Tour is coming to a city near you! Join our experts as they reveal how to enhance the way you design, analyze, sort, explore and discover.
Learn more »

Application Highlight

New white paper highlights life science applications

Life scientists today employ a wide range of cell analysis technologies to advance their research. Among them, multicolor flow cytometry offers the advantage of comprehensive, multiparametric analysis at the level of individual cells and subpopulations. With flow cytometry, as Figure 1 shows, we can look at the cell surface to analyze the expression of lineage and activation markers as well as cytokine and chemokine receptors. We can also detect intracellular molecules, such as cytokines or chemokines, signaling proteins or transcription factors and fluorescent proteins within the cytoplasm or the nucleus. Finally, we can examine cellular processes such as apoptosis, viability and proliferation.

App Highlight - Fig 1 Cell Diagram

Figure 1. Analyzing a cell by flow cytometry

A new BD white paper surveys flow cytometry applications across a broad range of life science disciplines, including immunology, cell biology, cancer research, stem cell research, molecular biology and microbiology. Almost all of its detailed data examples were collected and analyzed on the BD Accuri C6 Plus personal flow cytometer system, a compact, affordable, easy-to-use, transportable tool that can increase discovery in the lab or in the field. The following examples illustrate the rich data you can collect and analyze right on your benchtop.

App Highlight - Fig 2 Accuri Hero

Figure 2. The BD Accuri C6 Plus flow cytometer system
The instrument weighs just 13.6 kg (30 lb). Exterior dimensions (H x W x D) are 27.9 x 37.5 x 41.9 cm (11 x 14.75 x 16.5 in.), not including fluid tanks.

Immunology. Among the methodologies that contribute to immunology research, multicolor flow cytometry is preeminent. The white paper profiles two. First, it describes two different ways of analyzing cytokine expression: bead-based immunoassays (using BD™ Cytometric Bead Array, or CBA) and intracellular cytokine staining. Used together, these complementary approaches can provide a more comprehensive understanding of cytokine kinetics.

The paper also describes an advanced immunophenotyping technique called the Sentinel approach, which allows the detection of up to six markers using the four colors of the BD Accuri C6 Plus.

App Highlight - Fig 3 Jurkat Apoptosis Thumb
Figure 3. Time course of apoptosis in Jurkat cells
App Highlight - Fig 3 Jurkat Apoptosis Large
Figure 3. Time course of apoptosis in Jurkat cells

Jurkat cells were treated with 100 ng/mL of FAS ligand (Cat. No. 556375) to induce apoptosis, or with FAS ligand and BD Pharmingen™ Q-VD-OPh General Caspase Inhibitor (20 μM) or were left untreated. Cell viability was analyzed at 3, 6 and 24 hours. Cells were stained with BD Pharmingen Propidium Iodide Staining Solution (Cat. No. 556463) and BD Pharmingen APC Annexin V (Cat. No. 550474) and analyzed on the BD Accuri C6 Plus. Results: Over time, cells treated with FAS ligand (middle column) progressed from live to apoptotic to dead. The majority of cells treated with both FAS ligand and Q-VD-OPh (right column) remained viable, comparable to untreated cells (left column).

Cell and cancer biology. Using flow cytometry, cell and cancer biologists can study multiple cellular processes such as apoptosis, cell signaling, DNA content and damage, cell cycle and ploidy, proliferation, viability and calcium flux. The white paper includes a detailed example of a time-course study, illustrated in Figure 3, showing that FAS ligand induces apoptosis in Jurkat cells via caspase mediation. The BD Accuri C6 Plus makes time-course studies especially convenient because you can perform them right on the benchtop.

Flow cytometry is also a powerful tool for studying gene expression and regulation. The white paper demonstrates the ease and speed of optimizing a transfection protocol using the BD Accuri C6 Plus. We’ll profile this application in an upcoming issue of BD Accuri News.

Stem cell research. Monitoring the potency or differentiation of target cells is a primary challenge of stem cell research due to the high degree of cell heterogeneity as well as variable protocol efficiency. The BD white paper illustrates how you can assess pluripotency and differentiation by flow cytometry, using both cell surface and intracellular markers.

BD Stemflow™ kits have been designed and optimized for a variety of stem cell applications. Several of these kits are matched to free downloadable BD Accuri C6 Plus software templates to streamline the analysis of stem cell cultures.

Microbiology. Flow cytometry can provide data in a range of microbiological applications. Perhaps the most common microbial analysis task is to identify and count microbes. The unique fluidics system of the BD Accuri C6 Plus allows it to determine sample volume and count cells rapidly, directly and automatically from the software, eliminating laborious plate counts. The white paper demonstrates the use of fluorescence triggering to count total and viable bacteria in an E. coli sample treated with varying amounts of ethanol.

Compact size, rugged design and portability make the BD Accuri C6 Plus ideal for environmental research in the field and across the globe. The paper shows the analysis of three aquatic samples—coastal, bay and pond water—each with its own autofluorescence profile of microorganisms reflecting their relative production of chlorophyll, phycocyanin and phycoerithrin (PE).

Versatile, compact and affordable, the BD Accuri C6 Plus brings the power of flow cytometry within reach for individual research labs, small institutions and even core facilities. With its combination of performance and simplicity, the BD Accuri C6 Plus puts this essential cell analysis tool into the hands of more life science researchers than ever before.

For a limited time, take advantage of our new promotion that offers unbelievable savings on personal flow cytometry. Get a 35% discount on the purchase of a BD Accuri C6 Plus, or a 40% discount on the BD Accuri C6 Plus with the BD CSampler™ Plus automation accessory—both with an extra year of warranty.

Download the white paper »

Download the BD Accuri C6 Plus brochure »

Check out the promotion »


 

Tips & Tricks

White papers

Tips Tricks - White Papers Thumb

In addition to the new life sciences white paper described in this month’s Application Highlight, you can find an abundance of other BD Accuri™ white papers available at bdbiosciences.com. These white papers, often co-written by leading scientists in a field, delve deeply into a particular topic, including detailed data that shows how personal flow cytometry has led to new discoveries and insights. Topics include water quality, biofuels, bioprocess monitoring, platelet counting, aquatic microorganisms, intracellular calcium, plant DNA, cell analysis, direct counting, automation and utility for core facilities.

Browse our white papers »


 

Publication Picks

This section highlights interesting recent research articles using BD Accuri flow cytometers.

Stem cell signaling

Daneshvar K, Pondick JV, Kim BM, et al. DIGIT is a conserved long noncoding RNA that regulates GSC expression to control definitive endoderm differentiation of embryonic stem cells. Cell Rep. 2016;17:353-365. PubMed

Groundwater treatment

Besmer MD, Epting J, Page RM, Sigrist JA, Huggenberger P, Hammes F. Online flow cytometry reveals microbial dynamics influenced by concurrent natural and operational events in groundwater used for drinking water treatment. Sci Rep. 2016;6:3462. PubMed

CRISPR-Cas yeast editing

Reider Apel A, d’Espaux L, Wehrs M, et al. A Cas9-based toolkit to program gene expression in Saccharomyces cerevisiae. Nucleic Acids Res. 2016;9:496-508. PubMed

Wasp life cycle

Shimizu Y, Mukai M, Goto SG. Cell cycle arrest in the jewel wasp Nasonia vitripennis in larval diapause. J Insect Physiol. 2016;1910:30362-6. PubMed


 

Events

Meeting – June 10–14, 2017 – Boston, MA
CYTO 2017 (International Society for Advancement of Cytometry) »

Meeting – June 14–17, 2017 – Boston, MA
ISSCR 2017 (International Society for Stem Cell Research) »

BD Horizon Global Tour
Thursday, May 18, 2017 – Bethesda, MD (NIH) »
Wednesday, May 24, 2017 – Boston, MA »
Thursday, May 25, 2017 – Philadelphia, PA »
Thursday, June 1, 2017 – San Diego, CA »
Wednesday, June 7, 2017 – Seattle, WA »
Tuesday, June 20, 2017 – Atlanta, GA »
Thursday, June 22, 2017 – Chicago, IL »


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For Research Use Only. Not for use in diagnostic or therapeutic procedures.

1 All reagents and kits are compatible with both the BD Accuri C6 and the new BD Accuri C6 Plus flow cytometer systems. Data was generated on the BD Accuri C6 Plus. Information about BD reagent kits, BD Accuri C6 and BD Accuri C6 Plus software templates and BD CSampler™ and BD CSampler Plus automation options is available at www.bdbiosciences.com/accuri.