BD Accuri C6 Plus

Simplicity and Sensitivity on your Benchtop

The BD Accuri™ C6 Plus is equipped with a blue and red laser, two light scatter detectors, and four fluorescence detectors with optical filters optimized for the detection of many popular fluorochromes, including FITC, PE, PerCP-Cy™5.5, and APC, as well as newer polymer dyes such as BD Horizon Brilliant™ Blue 515. The BD Accuri C6 Plus can also analyze many variants of fluorescent proteins, such as GFP, YFP, and mCherry. Its adaptability and suitability for experiments up to four colors have allowed researchers to publish numerous scientific articles citing BD Accuri flow cytometers.

During manufacture, laser and optical alignments are set and locked down. With no need to adjust detector voltage, the system is easier to use. Data is digitally collected over a wide dynamic range and is fully available to users as needed, eliminating the risk of data loss due to incorrect settings. Software functions such as Zoom and VirtualGain™ allow visualization of data at any scale, so that users can precisely set gates and regions.

Despite its compact size, the optical system demonstrates extraordinary fluorescence sensitivity. Intensive testing ensures that the optical and fluidic design can withstand rugged conditions. Provided the system is anchored, it can run samples even if the benchtop is in motion, such as aboard a ship.

BD Accuri C6 Plus optical bench
A compact optical design, fixed alignment, and pre-optimized detector settings make the system easier to use.

Filter, laser, and configuration options provide added flexibility

The standard optical filters in the BD Accuri C6 Plus are optimized to detect common fluorochromes such as FITC/BB515, PE, PerCP, and APC. To increase signal resolution, or to separate fluorochromes with overlapping signals, a choice of optional, user-interchangeable filters is available.

BD Accuri C6 Plus fluorochrome support
All fluorochromes in the table can be detected by the BD Accuri C6 Plus equipped with standard filters. Optional filters can be used to increase resolution or to separate fluorochromes with overlapping signals, like GFP and YFP.

In the standard configuration of the BD Accuri C6 Plus, three detectors read fluorescence emissions from fluorochromes excited by the blue laser, while the fourth detector reads emissions from fluorochromes excited by the red laser (3-blue/1-red). By installing the optional Selectable Laser Module, you can operate the system in 2-blue/2-red and 4-blue configurations. This option significantly expands the fluorochrome combinations you can analyze.

Selectable Laser alternate configurations
The optional Selectable Laser Module allows two or four fluorescence channels to be assigned to the blue laser, vs three in the standard configuration. The remaining channels (if any) are assigned to the red laser.

Four-color whole blood immunophenotyping panel on the BD Accuri C6 Plus
Four-color whole blood immunophenotyping panel on the BD Accuri C6 Plus
Whole blood was stained with fluorescent antibodies to CD3, CD56, CD14, and CD19 and acquired and analyzed on a BD Accuri C6 Plus. Side scatter and CD14 expression were used to discriminate lymphocyte and monocyte populations. Within the lymphocyte gate, percentages of T cells, NKT cells, NK cells, and B cells were quantified based on expression of CD3, CD56, and CD19. In all, five blood cell populations were identified in a single tube using four colors.

Cy™ is a trademark of GE Healthcare.

All reagents and kits are compatible with both the BD Accuri C6 Plus and BD Accuri™ C6 flow cytometer systems. Platforms referred to as "BD Accuri" represent both the BD Accuri C6 Plus and BD Accuri C6. Data was generated on either the BD Accuri C6 Plus or the BD Accuri C6 as indicated in figure legends.