BD Accuri C6 Plus

Intuitive Software—Master in Minutes

BD Accuri™ C6 Plus software features an intuitive user interface. Even novice flow cytometry users find it so easy to learn and use that most can collect and analyze data in less than an hour. Software options and instrument controls are clearly visible from the software’s tabbed interface.


Data is acquired from the Collect tab. All tasks and settings are laid out simply on one screen for quick access and operation. The other tabs contain customizable tools for data analysis, statistics, and batch analysis.

Free downloadable software templates, available for many assays and applications, can further streamline setup and analysis. BD Accuri C6 Plus software files can be exported in FCS 3.1 format for seamless data import into flow cytometry analysis programs such as FCS Express™ software and FlowJo™ software.

Accuri C6 Plus - Analyze
Accuri C6 Plus - Statistics
Accuri C6 Plus - Batch Analysis

Automated instrument QC

The new Instrument QC feature automates the daily validation of the BD Accuri C6 Plus using BD™ Cytometer Setup and Tracking (CS&T) RUO beads to ensure that the instrument meets performance specifications. Instrument QC results are instantly displayed in the software and saved in PDF format. The software automatically generates Levey-Jennings plots that allow you to monitor instrument performance over time.


Instrument QC Compensation

After each time Instrument QC is performed, the software also updates compensation settings for FITC, PE, APC, and PerCP or PerCP-Cy™5.5. Instrument QC Compensation Settings can be selected from the Compensation Preferences window as an easy and convenient starting point for proper compensation controls.

Alternatively, you can select User Defined Compensation Settings to calculate compensation manually, which is also necessary for other fluorochromes or dyes.

Compensation settings remove artifacts of fluorescence spillover in the BD Accuri C6 Plus
Compensation settings remove artifacts of fluorescence spillover on the BD Accuri C6 Plus
Whole blood was analyzed for the expression of CD3 and CD19, two markers expressed differentially on lymphocytes and B cells, respectively. Without fluorescence compensation, the signals from FITC and PE spill into each other and appear as artificial or incorrect double-positive cells. After applying instrument QC compensation, the spillover effect is removed, and single positive CD3+ lymphocytes and CD19+ B cells can be readily distinguished.



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All reagents and kits are compatible with both the BD Accuri C6 Plus and BD Accuri™ C6 flow cytometer systems. Platforms referred to as "BD Accuri" represent both the BD Accuri C6 Plus and BD Accuri C6. Data was generated on either the BD Accuri C6 Plus or the BD Accuri C6 as indicated in figure legends.

BD Accuri C6 system (32-bit) software is not forward compatible with BD Accuri C6 Plus (64-bit) software and the FCS 3.1 file format.