BD LSRFortessa

Analysis of Regulatory T Cells

Analysis of Regulatory T Cells

Regulatory T cells (Tregs) play a critical role in maintaining immune modulation and are present in normal peripheral blood in low numbers (5 to 10% of CD4+ T cells or 1 to 2% of total lymphocytes). Forkhead box P3 (FoxP3) is considered to be the definitive marker for this rare cell population. Tregs are known to express the highest levels of CD25. The CD4+CD25+(high) gating strategy shown here was used to identify Treg populations. These plots represent data taken from a BD LSRFortessa using an 8-color panel that applies this gating strategy to identify Fox P3 positive cells.

Analysis of human blood
Analysis of human blood

Data shows a 10-color panel run on a 3-laser BD LSRFortessa with a blue, red, violet configuration. The panel was used for defining T-cell subsets using lysed whole blood (BD Pharm Lyse™ lysing buffer) from a normal donor. Use of the BD Horizon™ V450 and BD Horizon V500 dyes allows brighter fluorochromes to be used for more dimly expressed antigens, while the use of 10 colors expands the amount of information gained from a single tube.