Multicolor Flow Cytometry

Video Library

Watch our BD Horizon webinars and download the presentations from our experts as they reveal essential tips and considerations when performing multicolor flow cytometry. Choose from the nine subjects below:

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Quality flow cytometry data starts with proper panel design. Fluorochrome choices, instrument configurations/setup and knowledge of the biology being studied are important factors when considering panel choices.

In this module, we will provide you with a foundation to easily build the best possible panels to answer your experimental questions.

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A good panel is defined by its ability to resolve populations of interest. Matching bright fluorochromes to dim markers, and the ability to spread markers across multiple lasers, can minimize spectral overlap and maximize population resolution.

In the Analyze section, multiple data sets demonstrating how to maximize resolution of cell populations will be shared. Also included is an interactive session to review panels that you helped design.

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The ability to sort highly pure cell populations is fundamental to downstream biological analysis. An optimized panel is a key component of sort purity.

The Sort module reviews important considerations when you move from cell analysis to cell sorting. Information on how optimized panels improve sort outcomes will be discussed.

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The journey to “see what nature is hiding” using flow cytometry begins by understanding the biology of your cells and the relative brightness of fluorochromes. To maximize resolution, bright fluorochromes are used to resolve dim antigens. Enabling brighter fluorescence signals, BD Horizon Brilliant™ polymer dyes are perhaps the most significant advancement in reagent technology in a decade.


By understanding antigen density—the number of cell surface receptors on a cell—researchers can understand which antibody/fluorochrome combinations are candidates for panels.


Delivering information from multiple parameters simultaneously, a single flow cytometry experiment can speed the path to discovery. Traditionally, this is accomplished using many fluorochromes on a few lasers and subsequently addressing spectral overlap using compensation. The more colors used, the more overlap and the more complex the experiment.


Biology is seldom simple, but by leveraging new tools and methods, you can speed your journey to see nature more clearly. Our single goal at BD Biosciences is to equip you with the ability to be more efficient and effective in your research by making flow cytometry more powerful and easier to use.


Discover a new way of looking at fluorochromes and markers that can maximize the number of parameters achievable on any instrument.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.