BD FACSUITE SOFTWARE

Making global collaboration possible

BD FACSuite™ software provides an easy-to-use yet flexible interface for data acquisition and analysis with the BD FACSLyric™ system. The application is designed for user-defined assays and collaboration through the Assay Portability feature, and provides a simple, reproducible and transferrable setup. Instrument QC and setup are easy and fast.


Streamlined

FACSuite- Streamlined

BD™ CS&T Beads are used to check the cytometer performance and automatically make adjustments, ensuring consistent values from day to day and experiment to experiment. Comprehensive checks during system initialization and setup, including performance QC, verify system performance, ensure that detector settings are optimal and trigger the automated laser alignment process if required. The entire setup process can be performed without manual intervention.

Universal

Universal Setup ensures that setup is an easy, quick procedure for Instrument QC and Assay Setup for both IVD and user-defined assays. QC is shared between clinical and user-defined applications so you don't have to repeat your daily QC twice.

Time Saving

Measuring spillover in no longer a daily or weekly activity. On the BD FACSLyric flow cytometer, spillover value measurement is performed with pre-dispensed dried beads and reduced to once every 60 days. Spillover values are maintained within the system and updated automatically with PMTV changes.

FACSuite- Time-saving

Reproducible and Portable

FACSuite - Reproducibility

A key aspect of effectively using multicolor flow is ensuring that the experiment can be performed consistently over time and across instruments. Universal setup ensures consistency of results over time. The Assay Portability feature within BD FACSuite software enables consistency across multiple systems, ensuring reproducibility over time, across operators and across instruments.

Reproducible Across Instruments

To help enable reproducible results day to day, between instruments, or between sites, it is critical that cytometer setup is standardized and efficient. The BD FACSLyric system enables users to accurately adjust gains to achieve fluorescence (median fluorescence intensity, MFI) target values that are consistent across the instrument. Setup standardization between instruments or across sites helps enable equivalency between assays or experiments. When assay settings are imported across six BD FACSLyric cytometers, target MFI values show high reproducibility and a variance below 10%.

 
  Median Fluorescence Intensity
Instrument
Antigen Fluorochrome 1 2 3 4 5 6 %CV
CD4 FITC 1,891 1,699 1,816 1,865 1,661 1,691 5.6%
CD4 PE 16,781 16,781 17,393 18,363 16,993 17,482 3.5%
CD4 PerCP-Cy™5.5 4,615 4,847 4,546 5,012 4,661 4,913 3.9%
CD4 PE-Cy™7 25,402 27,600 26,066 29,213 30,510 26,393 7.2%
CD4 APC 20,064 21,163 20,768 20,600 20,990 18,848 4.2%
CD4 APC-R700 25,238 27,083 26,202 27,982 29,449 26,147 5.6%
CD4 APC-H7 7,406 7,662 7,607 8,000 8,119 7,634 5.6%
CD4 V450 5,234 5,220 5,473 5,299 5,631 5,581 3.3%
CD4 V500-C 2,294 2,332 2,338 2,354 2,318 2,039 5.2%

Between-instrument Reproducibility of Target MFI Values

Daily QC using one lot of BD CS&T beads was run on six 10-color BD FACSLyric cytometers. Target MFI values were defined and single-color stains of peripheral blood samples acquired on BD FACSLyric Instrument #1. Settings from Instrument #1 were imported onto, and the same set of stains acquired on, Instruments 2 through 6. The MFI of the relevant positive populations was measured for all parameters across all instruments. The %CV is shown.



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